HPLC Analysis of Polyphenoles in Coffee on Chromolith^® Performance 2 x 100 - 4.6mm

sample preparation

6g of coffee powder with 200ml of boiling water in a coffe-extractor.The extract was injected directly into the HPLC-System.

column

Chromolith Performance 100 x 4.6 mm (1.52028.0001)

mobile phase

[A] acetonitril; [B] water + 0,1% Acetic acid v/v;

gradient

Initial composition:0-3min 10%A, 3-14min 10%A to 50%A, hold 50%A until 20min

flow rate

2 mL/min

pressure

13369 psi 830-922 Bar

column temp.

ambient

detector

327 nm cell: 11 μL

injection

1.0 μL

sample

6g Coffee powder in 200ml Water