HPLC Analysis of Protein Standards on Zenix® SEC-300 at Low Flow Rate
材料
used together
产品编号
说明
价格
相关产品
产品编号
说明
价格
Zenix® SEC-300 Gel Filtration Column, 3 μm
L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steelCONDITIONS
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm (Z777033)
mobile phase
0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid
flow rate
0.1 mL/min
pressure
173 psi (12 bar)
column temp.
25 °C
detector
UV, 215 nm
injection
4 μL
sample
protein standards
说明
分析说明
Due to the low diffusion coefficents of proteins, optimal flow in size exclusion chromatography is often very low. However, the disadvantage is long run times. This example demonstrates both features.
法律信息
Zenix is a registered trademark of Sepax Technologies