HPLC Analysis of Protein Tryptic Digest on Ascentis^® RP-Amide

Ascentis RP-Amide, 25 cm x 2.1 mm I.D., 5 μm particles (565306-U)

[A] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:water; [B] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:acetonitrile

10 to 50% B in 80 min

0.2 mL/min

ambient

ESI (+)

4 μL

tryptic digest of carboxymethylated protein mixture 1.5 g/L in 50 mM formic acid

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany