HPLC Analysis of Proteins on TSKgel^® CM-5PW

column

TSKgel CM-5PW, 7.5 cm × 7.5 mm I.D., 10 μm particles (813068)

mobile phase

[A] 0.1 M sodium phosphate, monbasic buffer, pH 7.0; [B] 0.5 M sodium chloride, A to B in 60 min

flow rate

1 mL/min

detector

UV, 280 nm