HPLC Analysis of Rapamycin and 32-Desmethoxyrapamycin on Discovery^® HS C18

column

Discovery HS C18, 15 cm × 4.6 mm ID, 3μm particles (568522-U)

mobile phase

[A] 10 mM ammonium acetate, pH unadjusted: [B] acetonitrile (25:75, v/v, A:B)

flow rate

1.5 mL/min

column temp.

60 °C

detector

UV, 268 nm

injection

10 μg/mL