HPLC Analysis of Sigma Protein Standard Mix on SRT^® SEC-500

column

Sepax SRT SEC-500, 30 cm x 4.6 mm I.D., 5 μm particles (Z777055)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide

flow rate

0.3 mL/min

pressure

580 psi (40 bar)

temp.

25 °C

detector

UV, 215 & 280 nm

injection

2.5 μL

sample

0.5 mL of mobile phase added to one vial of  Sigma Protein Standard Mix (p/n 69385); filtered 0.45 μm Millex-HV syringe filter (p/n Z227552)