HPLC Analysis of Sigma Protein Standard Mix on Zenix^® SEC-300

column

Sepax Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)

mobile phase

0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide

flow rate

0.3 mL/min

pressure

1050 psi (72 bar)

column temp.

25 °C

detector

UV, 215 & 280 nm

injection

2.5 μL

sample

0.5 mL of mobile phase added to one vial of  Sigma Protein Standard Mix   (p/n 69385); filtered 0.45 μm Millex-HV syringe filter (p/n Z227552)