HPLC Analysis of Spironolactone on Chromolith^® HighResolution RP-18 endcapped 50 x 4.6 mm

column

Chromolith^® HighResolution RP-18 endcapped, 50 x 4.6 mm (1.52021.0001)

mobile phase

[A] water; [B] methanol; (40:60,:A:B)

flow rate

1.5 mL/min

pressure

856 psi (59 bar)

column temp.

ambient

detector

230 nm cell: 11μL

injection

7 μL (reduced with 67% as it is scaled to a 67% shorter column with same diameter)

sample

0.5 mg/mL, 500 ppm Spironolactone RS in diluent (diluent: acetonitrile/water 1:1 (v/v))