HPLC Analysis of Steroids on Ascentis^® Express C18

材料

T1500

睾酮

≥98%

53823-U

Ascentis^® Express C18, 2.7 μm HPLC 色谱柱

L × I.D. 10 cm × 2.1 mm, pk of 1 ea

E-058

表睾酮溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®

M-912

美雄酮溶液

1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant^®

N-050

诺龙溶液

1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®

N-914

Norethandrolone solution

1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant^®

CONDITIONS

column

Ascentis Express C18 10cm x 2.1mm, 2.7 μm particles (53823-U)

mobile phase

[A] water [B] acetonitrile (70:30, A:B)

flow rate

0.7 mL/min

pressure

5438 psi (375 bar)

column temp.

40 °C

detector

UV, 254 nm

injection

2 μL

sample

0.125 mg/mL each in 37.5/62.5 water/organic solvent

说明

一般描述

This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of 5 steroids.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Related Applications

Small Molecule HPLC Large Molecule HPLC Gas Chromatography (GC)TLC Solid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS

HPLC Analysis of Steroids on Ascentis® Express C18

材料

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