HPLC Analysis of Steroids on Ascentis^® RP-Amide (Mobile phase: 60:40)

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] water; [B] acetonitrile; (60:40, A:B)

flow rate

1 mL/min

column temp.

35 °C

detector

UV, 254 nm

injection

10 μL

sample

50 μg/mL each in mobile phase