HPLC Analysis of Substituted Aromatic Compounds on Ascentis^® Express Biphenyl

Ascentis Express Biphenyl, 10 cm x 2.1 mm, 2.7 μm particles (64065-U)

[A] Water; [B]Methanol; (57:43 A:B)

0.5 mL/min

7900 psi (545 bar)

35 °C

UV, 254 nm

1 μL

Uracil (25 μg/mL), Benzonitrile (50 μg/mL), Acetophenone (25 μg/mL), Anisole (100 μg/mL),

This application demonstrate the separation of substituted aromatic compounds on Ascentis Express Biphenyl. These analytes highlight the unique selectivity of this stationary phase for this type of analytes using methanol reversed phase conditions.

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany