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HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95)

HPLC Analysis of Sugars on Ascentis® Express HILIC (Mobile Phase 5:95) application for HPLC

材料

相关产品

产品编号
说明
价格

C7252

D-(+)-纤维二糖

≥98% (HPLC)

A3256

L -(+)-阿拉伯糖

≥99% (GC)

84097

蔗糖

BioUltra, Molecular Biology, ≥99.5% (HPLC)

53981-U

Ascentis® Express HILIC, 2.7 μm HPLC色谱柱

2.7 μm particle size, L × I.D. 15 cm × 4.6 mm

1287700

Galactose

United States Pharmacopeia (USP) Reference Standard

G0350500

D -(+)-葡萄糖

European Pharmacopoeia (EP) Reference Standard

X0200000

D-(+)-木糖

European Pharmacopoeia (EP) Reference Standard

1375182

甘露糖

United States Pharmacopeia (USP) Reference Standard

CONDITIONS

column

Ascentis Express HILlC, 15 cm x 4.6 mm I.D., 2.7 μm particles (53981-U)

mobile phase

[A] water; [B] acetonitrile; (5:95, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

ELSD, 55 °C, 3.5 bar nitrogen

injection

10 μL

说明

分析说明

This application demonstrates the suitability of the Ascentis Express HILIC for the analysis of sugars. Structures along with the optimized chromatogram obtained on the Ascentis Express HILIC are presented. Separation is by normal-phase mechanism as a decrease in mobile phase water content results in increased retention

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Chromatogram Search

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Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS