HPLC Analysis of Toluidine Isomers on Ascentis^® Si (Hexane: 2-Propanol)

column

Ascentis Si, 15 cm × 4.6 mm I.D., 5 μm particles (581512-U)

mobile phase

[A] hexane; [B] 2-propanol; (96:4, A:B)

flow rate

1.0 mL/min

column temp.

35 °C

detector

UV, 245 nm

injection

10 μL

sample

50 μg/mL in mobile phase