HPLC Analysis of Tryptic Digest of Protein Mixture on BIOshell^™ A160 2.7 μm Peptide C18

column

BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)

mobile phase

[A] 0.1% formic acid in water; [B] 0.4% formic acid in 25:75 (v/v) water:acetonitrile

gradient

6 to 70% B in 40 min

flow rate

0.3 mL/min

column temp.

35 °C

detector

ESI(+), TOF

injection

2 μL

sample

10 pmol/L in 0.1% formic acid