HPLC Analysis of Veterinary Antibiotics on Ascentis® C18

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CONDITIONS
column
Ascentis C18, 5 cm x 2.1 mm I.D., 3 μm particles (581300-U)
mobile phase
[A] 50 mM ammonium acetate (pH 6.0): [B] methanol; (20:80, A:B)
flow rate
0.2 mL/min
column temp.
22 °C
detector
ESI(-), full scan
injection
2 μL
sample
as indicated in 50:50, water:methanol
说明
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany