HPLC Analysis of Veterinary Antibiotics on Ascentis^® C18

column

Ascentis C18, 5 cm x 2.1 mm I.D., 3 μm particles (581300-U)

mobile phase

[A] 50 mM ammonium acetate (pH 6.0): [B] methanol; (20:80, A:B)

flow rate

0.2 mL/min

column temp.

22 °C

detector

ESI(-), full scan

injection

2 μL

sample

as indicated in 50:50, water:methanol