HPLC Analysis of Vitamins B1, B6 and B12 using Ascentis® Express RP-Amide
材料
used together
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Thiamine HCl (Vitamin B1) solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Cyanocobalamin (Vitamin B12) solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 5 μm particles (50773-U)
mobile phase
[A] 0.02 M Ammonium Formate, pH = 3.0; [B] acetonitrile
gradient
0-50% B in 1 min. Hold at 50% B until 1.5 min
flow rate
1.5 mL/min
pressure
1639 psi (113 bar)
column temp.
30 °C
detector
UV, 276 nm
injection
1 μL
sample
Dissolved in 50/50 water/ACN
说明
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany