HPLC Analysis of Vitamins, Water Soluble, on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

25 mM potassium phosphate, dibasic (pH 3.5 with phosphoric acid)

flow rate

1.0 mL/min

column temp.

30 °C

detector

UV, 230 nm

injection

10 μL

sample

as indicated in 25 mM potassium phosphate, dibasic (pH 3.5 with phosphoric acid)