HPLC Analysis of Vitamins, Water Soluble, on Discovery^® RP-Amide C16

column

Discovery RP-Amide C16, 15 cm × 4.6 mm I.D., 5 μm particles (505013)

mobile phase

[A] methanol; [B] 50 mM potassium phosphote, monobasic, pH 4.5 (30:70, A:B)

column temp.

35 °C

detector

UV, 220 nm

injection

10 μL