HPLC Analysis of Voriconazole With Basic Stress on Chromolith^® Performance 100-4.6 mm

sample preparation

stirring 0.5 hour in NaOH, 0.1N

column

Chromolith^® Performance 100 x 4.6 mm (1.52028.0001)

mobile phase

[A] acetonitrile; [B] water; (50:50, A:B)

flow rate

1 mL/min

pressure

4758 psi (33 bar)

column temp.

ambient

detector

256 nm cell: 11 μL

injection

20 μL

sample

Voriconazole 50 μg/mL