HPLC Size-Exlusion Analysis of mAb Monomer, Dimer, and Higher-Order Aggregates on TSKgel® SuperSW mAb HTP
CONDITIONS
column
TSKgel SuperSW mAb HTP 4 μm, 15 cm x 4.6 mm I.D., 4 μm particles (822855)
mobile phase
0.2 M phosphate buffer, pH 6.7 + 0.05% sodium azide
flow rate
0.5 mL/min and 0.35 mL/min
column temp.
25 °C
detector
UV, 280 nm
injection
5 μL
sample
Monoclonal antibody 2 (mouse-human chimeric IfG, Erbitux)
说明
分析说明
Size-exclusion chromatography is now routinely used in the quality control of commercial monoclonal antibody (mAb) preparations. In this example, a mAb monomer, dimer and higher order aggregates are easily separated within 5 minutes on a 15 cm x 4.6 mm ID TSKgel® SuperSW mAb HTP column at a high flow rate of 0.35 mL/min. After Increasing the flow rate to 0.5 mL/min the monomer elutes within 3 minutes from the column.
法律信息
TSKgel is a registered trademark of Tosoh Corporation