Kromasil Eternity C18, 10 cm x 3.0 mm I.D., 2.5 μm particles (K0897111)
[A] 10 mM ammonium acetate in water:methanol (95:5); [B] 10 mM ammonium acetate in water:methanol (10:90)
0% B held for 1 min; to 100% B in 8.5 min;100% B held for 1 min
0.4 mL/min
332 psi (23 bar)
45 °C
MS, ESI(+) TOF, EIC
5 μL
100 ng/mL in mobile phase A
Nicotine and metabolites are shown here separated on a Kromasil Eternity C18 column. High purity LC/MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant CRMs provided reliable identification and quantification.
Eternity is a trademark of EKA Chemicals AB
Kromasil is a registered trademark of EKA Chemicals AB