LC/MS Analysis of Peptides, Hydrophobic, on Ascentis^® C18

column

Ascentis C18, 10 cm x 2.1 mm I.D., 5 μm particles (581326-U)

mobile phase

[A] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:water; [B] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:acetonitrile

gradient

20 to 50% B in 15 min

flow rate

0.2 mL/min

column temp.

ambient

detector

ESI (+)

injection

4 μL

sample

as indicated in 10 mM formic acid