LC/MS Analysis of Sudan Red Dyes on Ascentis^® RP-Amide

column

Ascentis RP-Amide, 15 cm × 4.6 mm I.D., 5 μm particles (565324-U)

mobile phase

[A] 0.1% formic acid in water (pH unadjusted): [B] acetonitrile; (15:85, A:B)

flow rate

1 mL/min, split to the MS

column temp.

35 °C

detector

MS, APCI (+) in selected ion recording (SIR) mode

injection

15 μL

sample

1 μg/mL in 30:70, 0.1% formic acid in water (pH unadjusted):acetonitrile