Ascentis Express RP-Amide 10cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
[A] 20 mM Ammonium acetate pH 4.0 with Acetic Acid; [B] ACN; (85:15, A:B)
15% B for 2 min; 15 to 30% B in 6 min, held at 30% B for 4 min
0.6 mL/min
4757 psi (328 bar)
35 °C
UV, 280 nm
5 μL
25C-NBOMe HCl, 25B-NBOMe HCl, 25I-NBF HCl, 25INBMD HCl, 25I-NBOME HCl, 20 μg/mL in 90:10 mobile phase A:mobile phase B
This application demonstrates the suitability of Ascentis Express RP-Amide for the HPLC separation of substituted phenethylamine psychedelics (Bath Salts). This application shows the use of UV detection for this set of compounds.
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