Scalability of Separations on SeQuant^® ZIC-HILIC. Analytical to Semi-Preparative Scale

column

SeQuant ZIC-HILIC, PEEK, 50, x 2.1 mm, 5 μm, 200 Å (<href="/ProductLookup.html?ProdNo=150450&Brand=MM">1.50450.0001,<href="/ProductLookup.html?ProdNo=150451&Brand=MM">1.50451.0001 &Brand=MM">1.50451.0001)

mobile phase

[A] acetonitrile; [B] 5 mM ammonium acetate; (80:20, A:B)

flow rate

0.1, 0.5, 1.3, 9.3 mL/min

pressure

144, 144, 115, 158 psi (1.0, 1.0, 0.8, 1.1 MPa)

detector

254 nm

injection

1, 5, 20, 100 μL