USP HPLC Analysis of Carbamazepine on an Ascentis^® Express ES-CN Column

column

Ascentis Express ES-Cyano, 10 cm x 4.6 mm I.D., 2.7 μm particles (53491-U)

mobile phase

(A)water, (B)methanol, (C)THF; (850:120:30, A:B:C) and 0.22 mL formic acid, 0.5 ml triethylamine per liter

flow rate

1.25 mL/min

pressure

3480 psi (240 bar)

column temp.

30 °C

detector

UV, 230 nm

injection

20 μL

sample

0.2 mg/mL in water:methanol (1:1)