HybridSPE-Precipitation 96-well Plate, 50 mg/well (575656-U)
SPE (Solid Phase Extraction)
Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 μm particles (53822-U)
Rat plasma spiked with verapamil and metabolites at 10 ng/mL
[A] water; [B] acetonitrile, pH 2.55 adjust with formic acid (30:70, A:B)
0.6 mL/min
35 °C
TOF/MS
1 μL
100 μL spiked rat plasma followed by 300 μL 1% formic acid in acetonitrile
apply vacuum
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
HybridSPE is a registered trademark of Merck KGaA, Darmstadt, Germany