LC/MS Analysis of 3-Methoxytyramine and Metanephrines on SeQuant^® ZIC-HILIC

column

SeQuant ZIC-HILIC, 10 cm x 2.1 mm, 3.5 μm, 100 Å particles (1.50441.0001)

mobile phase

5 mM ammonium formate with 0.1% formic acid in 85:15, acetonitrile:water

flow rate

0.5 mL/min

pressure

3500 psi (241 bar)

column temp.

25 °C

detector

MS, ESI+, combined SIR

injection

1 μL

sample

200 ng/mL in acetonitrile