CN
LC/MS Analysis of Annato (or Roucou or Achiote) on Chromolith® FastGradient RP-18 endcapped
材料
流动相组分
产品编号
说明
价格
CONDITIONS
column
Chromolith FastGradient RP-18, 5 cm x 2.0 mm I.D, 2 μm particles (1.52007.0001)
mobile phase
[A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient
55 to 64% B in 0.5 min, to 70% B in 3 min, to 90% B in 0.5 min, held at 90% B for 2 min, to 55% B in 0.5 min, held at 55% for 2.5 min
flow rate
0.4 mL/min
pressure
562-317 psi (39 - 22 bar)
column temp.
25 °C
detector
ESI-MS(+) (m/z range 378-396), BPC
injection
10 μL
说明
其他说明
Sample Prep: Dissolve 2.2 mg/mL cis-norbixin and 0.12 mg/mL cis-bixin in diluent. After dissolution of the analytes the solution was heated for 5.5 hr at 75 °C under reflux to speed up transformation of cis- to trans-isomers and thereafter diluted 1:10 with acetonitrile and water (50:50 v/v).
法律信息
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany