LC/MS Analysis of Antidepressants From Urine Using an Ascentis® Express C18 Column
材料
相关产品
Citalopram hydrobromide solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Duloxetine hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Milnacipran hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Sertraline hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Trazodone hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®Venlafaxine hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis Express C18, 10cm x 2.1mm, 2.7 μm particles (53823-U)
column
Ascentis Express C18, 10cm x 2.1mm, 2.0 μm particles (50813-U)
mobile phase
[A] 5 mM ammonium acetate in 0.1% formic acid; [B] 0.1% formic acid in acetonitrile
gradient
10% B held for 1 min; to 55% B in 10 min
flow rate
0.4 mL/min
pressure
8280 psi (570 bar)
column temp.
35 °C
detector
MS, ESI(+), MRM, m/z 247.138/71.958, 278.168/58.025, 372.054/148.011, 442.114/155.035, 298.014/43.96, 305.968/158.873, 325.128/108.95
injection
1 μL
说明
一般描述
Sample preparation: Prepare a stock solution with 100 ng/mL of compounds in synthetic urine (Cerilliant, part number S-020, lot 72112). Dilute this solution with 95:5 (5mM ammonium acetate in 0.1% formic acid in water: 0.1% formic acid in acetonitrile) to give a final concentration of 50 ng/mL. Filter through a Millex LH filter. Inject. Also prepared blank in same way.
分析说明
Seven modern antidepressants were analyzed from urine after a dilute and inject method using reversed-phase chromatography and mass spectrometric detection.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany