LC/MS Analysis of Citicoline on SeQuant^® ZIC-HILIC

column

SeQuant^® ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100 Å (1.50661.0001)

mobile phase

[A] acetonitrile; [B] 7.7 g ammonium acetate and dissolve in in 1L MilliQ water; (70:30, A:B)

flow rate

0.75 mL/min

pressure

856 psi (59 bar)

column temp.

30 °C

detector

276 nm

injection

20 μL

sample

Weigh 25 mg citicoline in 50 ml volumetric flask. Dissolve in diluent.