Ascentis Express RP-Amide, 10 cm x 2.1 mm I.D., 2.7 μm particles (53913-U)
0.1% (v/v) formic acid in water
0.4 mL/min
3066 psi (211 bar)
35 °C
MS, ESI(-), combined XIC, 125.0 m/z, 221.1 m/z
2 μL
2 μg/mL in 99:1, water:methanol
The compounds ethyl sulfate and ethyl-β-D-glucuronide are metabolites of ethanol. Their analysis is of clinical interest as biomarkers or indicators of alcohol-induced liver disease (ALD). The HPLC analysis of these compounds using reversed-phase mode using conventional C18 chemistries suffers from poor retention due to the compounds′ polar nature. However, polar compound retention is improved by using the Ascentis Express RP-Amide, a polar-embedded phase, while still keeping the reversed-phase mechanism. Cerilliant CRMs provided reliable quantification.
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany