LC/MS Analysis of Ethanol Metabolites in Urine on Ascentis® Express OH5 after Dilution with Deuterated Internal Standards
材料
分析色谱柱
标准
乙基-β-D-葡萄糖醛酸标准液
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®乙基-β-D-葡萄糖醛酸苷-(乙基-d5)
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®乙基硫酸钠标准液 钠盐
1.0 mg/mL in methanol (as ethyl sulfate), ampule of 1 mL, certified reference material, Cerilliant®乙基硫酸钠盐-d5标准液 CRM 钠盐
1.0 mg/mL in methanol (as ethyl sulfate), ampule of 1 mL, certified reference material, Cerilliant®流动相组分
CONDITIONS
column
Ascentis Express OH5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53757-U)
mobile phase
[A] 5 mM ammonium formate and 0.1% formic acid in 95:5, acetonitrile:water; [B] 5 mM ammonium formate and 0.1% formic acid in 80:20, acetonitrile:water
gradient
0 to 100% B in 1 min, held at 100% B for 4 min, to 0% B, held for 5 min
flow rate
0.4 mL/min
pressure
1450 psi (100 bar)
column temp.
30 °C
detector
MS, ESI(-), 125.0 m/z, 126.1 m/z, 221.1 m/z, 226.2 m/z
injection
5 μL
sample
urine spiked with ethyl sulfate and ethyl-ß-D-glucuronide, both at 50 ng/mL, diluted 20:1 in acetonitrile containing deuterated internal standards at 100 ng/mL
说明
一般描述
Ethyl sulphate (EtS) and ethyl glucuronide (EtG) are direct ethanol metabolites and may indicate recent alcohol consumption. The two compounds differ in their pathways for formation and degradation. Being polar compounds, they are poorly retained by C18 phases and elute early in the chromatogram along with matrix compounds. This leads to poor or unreliable quantification. The method shown here uses HILIC mode on an Ascentis Express OH5 column to retain both analytes well, making it very likely to be more robust and reliable, as well as highly MS-friendly. Cerilliant CRMs provided reliable quantification. The internal standard was needed for accurate quantification. External calibration resulted in a large excess of sulfate.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany