SPE (Solid Phase Extraction)
urine, 1 mL
Supel-Select SCX SPE 96-well Plate, 30 mg/well (575664-U)
1 mL methanol, then 1 mL 0.1% formic acid in water
2 x 1 mL 10% methanol in water, 2 x 1 mL 1% formic acid in methanol
2 mL 5% ammonium hydroxide in acetonitrile
evaporate to 500 μL with nitrogen at room temperature, reconstitute in 250 μL acetonitrile
Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
5 mM ammonium formate in acetonitrile:water (90:10, v/v)
0.4 mL/min
1570 psi (108 bar)
35 °C
TOF/MS
2 μL
The fluoroamphetamines (FA) are a group of "designer" stimulant drugs in the amphetamine family. Four members of the FA family are shown here isolated from urine using Supel-Select SCX SPE and separated in under 3 minutes on an Ascentis Express HILIC column. Cerilliant CRMs were used to ensure reliable MS identification and quantification.
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