LC/MS Analysis of Meglumine on SeQuant^® ZIC-HILIC

column

SeQuant^® ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100Å (1.50661.0001)

mobile phase

[A] acetonitrile; [B] 50 mM ammonium acetate, adjusted with acetic acid to pH 5.0; (65:35, A:B)

flow rate

1.2 mL/min

pressure

1479 psi (102 Bar)

column temp.

45 °C (oven) (45 °C (detector cell))

detector

Refractive Index (RI) (cell: 10 μL)

injection

10 μL

sample

Dissolve 25 mg of meglumine standard in 10 mL of diluent (Diluent: Mobile phase)