LC/MS Analysis of Metformin and Related Impurities on SeQuant^® ZIC-HILIC

column

SeQuant^® ZIC-cHILIC, PEEK, 150 x 4.6 mm, 3 μm, 100 Å (1.50661.0001)

mobile phase

[A] acetonitrile; [B] 4.62 g of ammonium acetate in 1000 ml water (60 mM). [A] acetonitrile; [B] 4.62 g of ammonium acetate in 1000 ml water (60 mM). Adjust buffer to pH 5 using glacial acetic acid; (90:10, A:B)

flow rate

1.5 mL/min

pressure

1522 psi (105 bar)

column temp.

30 °C

detector

218 nm cell: 8 μL

injection

5 μL

sample

5000 ppm metformin & 1 ppm of each impurity: A, C and melamine & 5 ppm of impurity B in mobile phase