LC/MS Analysis of Nucleotides on SeQuant^® ZIC-cHILIC. Effect of Mobile Phase Buffer Concentration

column

SeQuant ZIC-cHILIC, 10 cm x 2.1 mm, 3.0 μm particles (< href="/ProductLookup.html?ProdNo=150657&Brand=MM">1.50657.0001)

mobile phase

[A] acetonitrile; [B] 50 mM or 100 mM ammonium acetate, pH 5.3 with acetic acid in water

gradient

26 to 27% B in 10 min; to 27 to 35% B in 10 min; to 26% B in 0.1 min; hold at 26% B for 4.9 min

flow rate

0.3 mL/min

pressure

1125 psi (77 bar)

column temp.

40 °C

detector

Q-TOF (ESI-negative), XIC of the following m/z values: AMP-346, ADP-426, ATP-506, CMP-322, CDP-402, CTP-482, GMP-362, GDP-442, GTP-522, UMP-323, UDP-403, UTP-483

injection

10 μL

sample

10-30 μg/mL of nucleotides (AMP, ADP, ATP, CMP, CDP, CTP, GMP, GDP, GTP, UMP, UDP, UTP) (80/20 acetonitrile/50 mM ammonium acetate in water, pH 5.3 with acetic acid)