LC-MS Analysis of Sterols with Ascentis® Express C18
材料
used together
相关产品
Aldosterone solution
100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®Corticosterone solution
1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®17α-Methyltestosterone solution
1.0 mg/mL in 1,2-dimethoxyethane, ampule of 1 mL, certified reference material, Cerilliant®Testosterone solution
1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
column
Ascentis® Express 90 Å C18, 2.7 µm, 10 cm x 2.1 mm, (53823-U)
mobile phase
[A]Water, 5 mM ammonium formate, 0.1% formic acid, pH 4.0; [B] Methanol
gradient
0-3 min 60% B, 3-5 min to 9% B, 5-7.5 min 95% B, 7.5-7.51 to 60% B, 7.51-11 min 60% B
flow rate
0.3 mL/min
pressure
190 bar
column temp.
50 °C
detector
MS/MS +ESI/ -ESI; spray voltage: 3.0 kV; nebulizing gas: 2 L/min; drying gas: 15 L/min; DL temp: 250 ˚C; heat block: 400 ˚C; MRM transitions see Analysis Notes
injection
2 µL
sample/matrix
Steroid standards of 10 ng/mL in 45% methanol and 55% water
说明
一般描述
Synthetic hormones and steroids have found application in the food industry, and many animals in the food chain have been exposed to high levels of steroids during their lifetime. Steroid hormones are naturally occurring, lipophilic compounds, that regulate many essential functions in the human body. Increased exposure to high levels of steroids, however, has been linked to cardiovascular system damage and cancer. Steroids, which are typically used as growth promoters and those used for therapeutic purposes, were screened in beef samples.
分析说明
MRM Transitions: Aldostrerone 361.0000>343.1000, Corticosterone 347.6000>109.0000, α-Zearalanol 321.0000>277.0000, Testosterone 289.0000>109.0000, 17α-Methyltestosterone 303.1000>97.0000, and Progesterone 315.0000>109.1000
其他说明
APP_573
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany