SeQuant ZIC-cHILIC, 10 cm x 2.1 mm, 3.0 μm, (1.50657) (< href="/ProductLookup.html?ProdNo=150657&Brand=MM">1.50657.0001)
[A] acetonitrile; [B] 50 mM ammonium acetate, pH 5.3 with acetic acid in water
26 to 27% B in 10 min; to 27 to 35% B in 10 min; to 26% B in 0.1 min; hold at 26% B for 9.9 min
0.3 mL/min
653 psi (35 °C), 986 psi (40 °C), 1030 psi (45 °C), 1088 psi (50 °C), 1117 psi (55 °C) (45 bar (35 °C), 68 bar (40 °C), 71 bar (45 °C), 75 bar (50 °C), 77 bar (55 °C))
as indicated (35 - 55 °C)
Q-TOF (ESI-negative), XIC of the following m/z values: AMP-346, ADP-426, ATP-506, CMP-322, CDP-402, CTP-482, GMP-362, GDP-442, GTP-522, UMP-323, UDP-403, UTP-483
10 μL
10-30 μg/mL of nucleotides (AMP, ADP, ATP, CMP, CDP, CTP, GMP, GDP, GTP, UMP, UDP, UTP) (80/20 acetonitrile/50 mM ammonium acetate in water, pH 5.3 with acetic acid)
This analysis demonstrates the polar nucleotides run under HILIC conditions on the phosphorylcholine zwitterionic phase (SeQuant ZIC-cHILIC) show an increase in retention and pressure as you increase the temperature.
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