LC/MS (TOF) Analysis of 2C Amines Drugs of Abuse in Urine on Ascentis® Express HILIC after Solid Phase Extraction (SPE) using Supel™-Select SCX
材料
SPE 管或板
分析色谱柱
标准
4-Bromo-2,5-dimethoxyphenethylamine hydrochloride solution
1.0 mg/mL in methanol (as free base), ampule of 1 mL, certified reference material, Cerilliant®2C-B-FLY hydrochloride solution
1 mg/mL in acetonitrile: water (9:1), ampule of 1 mL, certified reference material, Cerilliant®2,5-Dimethoxy-4-(n)-propylthiophenethylamine hydrochloride solution
1.0 mg/mL in acetonitrile: water (9:1) (as free base), ampule of 1 mL, certified reference material, Cerilliant®CONDITIONS
sample preparation
SPE (Solid Phase Extraction)
sample/matrix
spiked urine, 1 mL
SPE well plate
Supel-Select SCX SPE 96-well Plate, 30 mg/well (575664-U)
condition
1 mL methanol, then 1 mL 0.1% formic acid in water
washing
2 x 1 mL 10% methanol in water, 2 x 1 mL 1% formic acid in methanol
elution
2 mL 5% ammonium hydroxide in acetonitrile
eluate post-treatment
evaporate to 500 μL with nitrogen at room temperature, reconstitute in 250 μL acetonitrile
column
Ascentis Express HILIC, 10 cm x 2.1 mm I.D., 2.7 μm particles (53939-U)
mobile phase
5 mM ammonium formate in acetonitrile:water (90:10, v/v)
flow rate
0.4 mL/min
pressure
1570 psi (108 bar)
column temp.
35 °C
detector
TOF/MS
injection
2 μL
说明
分析说明
The 2C amines are a family of illicit, psychedelic phenethylamines. The term "2C" refers to the two carbon atoms between the benzene ring and the amino group in the parent molecular structure. A selection of 2C amines is shown here extracted from urine using Supel-Select SCX polymeric SPE, separated on an Ascentis Express HILIC column, and detected by TOF-MS. Cerilliant CRMs were used to ensure reliable MS identification and quantification.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Supel is a trademark of Sigma-Aldrich Co. LLC