coated tip
HPTLC pre-coated plate RP-18 wF 254s (1.13124.0001)
[A] methanol; [B] buffer solution, pH 7; [C] water; (60:20:20, A:B:C)
7 cm
normal chamber without chamber saturation
0.2 μL
in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm
dipped in dichlorofluorescein (0.2% in ethanol)