TLC Analysis of DNP Amino Acids on HPTLC CN F254s (analyte set 3)

材料
配件
产品编号
说明
价格
Hamilton® 薄层色谱注射器
1701, volume 10 μL, needle size 26s ga, needle type, fixed, needle L 51 mm (2 in.),coated tip
CONDITIONS
plate
HPTLC pre-coated plate CN F254s (1.16464.0001)
eluent
[A] acetone; [B] methanol; [C] water; (45:10:45, A:B:C) including 0.1 M lithium chloride
migration distance
8 cm
chamber
normal chamber without chamber saturation
sample
0.4 μL
detector
dipped in dichlorofluorescein (0.2% in ethanol) (in situ evaluation with TLC/HPTLC scanner (Camag) UV 254 nm)