Ascentis Express RP-Amide, 10 cm x 3.0 mm I.D., 2.7 μm particles (53918-U)
[A] 20 mM potassium phosphate, pH 3; [B] methanol
held at 0.5% B for 1.5 minutes, to 25% B in 1.0 minutes, held at 25% B for 1.7 minute, held at 0.5% B for 1.2 minutes
0.75 mL/min
5584 psi (385 bar)
30 °C
UV, 210 nm
0.5 μL
vitamin water was filtered through 0.2um nylon syringe filter
The vitamin water drink was filtered using 0.2 um Nylon filter and injected into UHPLC for B-vitamin analysis
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany