UHPLC Analyis of Protein Standards on Sepax Unix™ SEC-300: Column Performance as a Function of Flow Rate
材料
相关产品
CONDITIONS
column
Sepax Unix SEC-300, 15 cm x 4.6 mm I.D., 1.8 μm particles (Z777300)
mobile phase
0.2 M sodium phosphate, dibasic, pH 7.0 with phosphoric acid
flow rate
0.1 mL/min
flow rate
0.3 mL/min
flow rate
0.5 mL/min
pressure
500 psi (34 bar)
pressure
1550 psi (107 bar)
pressure
2400 psi (165 bar)
column temp.
25 °C
detector
UV, 215 nm
injection
2 μL
sample
Waters BEH200 SEC protein standard
说明
分析说明
In considering the capabilities of modern analytical HPLC and UHPLC instrumentation and columns, column performance (as measured by peak efficiency) signifcantly declines as flow rate increases, in the case of size exclusion chromatography of proteins. Despite this universal phenomenon, due to the slow diffusion coefficients biomacromolecules, it is remarkable that resolution can remain more than adequate at the higher flow rates, and thus allow for dramatic gains in throughput, particularly with small-particle columns.
法律信息
Unix is a trademark of Sepax Technologies