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UHPLC Analysis of a Protein Standard on TSKgel® UP-SW3000: Speed Advantage

UHPLC Analysis of a Protein Standard on TSKgel® UP-SW3000: Speed Advantage application for UHPLC

材料

相关产品

产品编号
说明
价格

818675

TSK-GEL® 体积排阻（SW 类型）高效液相色谱柱

phase diol, L × I.D. 30 cm × 4.6 mm, 4 μm particle size

CONDITIONS

column

TSKgel UP-SW3000, 15 cm x 4.6 mm I.D., 2.0 μm particles

column

TSKgel SuperSW3000, 30 cm x 4.6 mm I.D., 4.0 μm particles (818675)

mobile phase

0.2 M sodium phosphate, dibasic, pH 7.0 with phosphoric acid

flow rate

0.3 mL/min

pressure

1900 UP-SW3000 (131 bar)

pressure

1280 SuperSW3000 (88 bar)

column temp.

25 °C

detector

UV, 215 nm

injection

1 μL (UP-SW3000)

injection

2 μL (SuperSW3000)

sample

protein standard mix

说明

分析说明

A protein standard was chromatographed on a TSKgel® UP-SW3000, 15 cm x 4.6 mm I.D., 2.0 μm and TSKgel SuperSW3000, 30 cm x 4.6 mm I.D., 4.0 μm to compare the speed of analysis and performance on shorter column length. In comparison to the SuperSW3000, 4 μm column, the UP-SW3000, 2.0 μm column provides greater than or equal the efficiency, in half the time.

法律信息

TSKgel is a registered trademark of Tosoh Corporation

Chromatogram Search

Related Product Categories

HPLC ColumnsGC Columns & AccessoriesTLC Plates & AdsorbentsSolid Phase Microextraction (SPME)Solid Phase Extraction & QuEChERS

Related Applications

Small Molecule HPLCLarge Molecule HPLCGas Chromatography (GC)TLCSolid Phase Microextraction (SPME)Solid Phase Extraction (SPE)QuEChERS