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UHPLC Analysis of Aldehyde and Ketone DNPH Derivatives on Ascentis® Express C18

UHPLC Analysis of Aldehyde and Ketone DNPH Derivatives on Ascentis® Express C18 application for UHPLC

材料

分析色谱柱

产品编号
说明
价格

Ascentis® Express C18, 2 μm UHPLC 色谱柱

2 μm particle size, L × I.D. 10 cm × 2.1 mm

CONDITIONS

column

Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 μm particles (50813-U)

mobile phase

[A] water; [B] acetonitrile; (40:60, A:B)

flow rate

0.8 mL/min

pressure

9520 psi (656 bar)

column temp.

45 °C

detector

UV, 360 nm

injection

0.1 μL

sample

CARB Method 1004 DNPH Mix 2 (47651-U) diluted 10:1 in 40:60, water:acetonitrile

说明

分析说明

Environmental contaminants analyzed by this UHPLC separation are a standard mix of aldehydes and kethones as their DNPH derivatives. Formaldehyde is the target compound for US EPA Compendium Method TO-11A and Method 8315, but at least twelve other carbonyl compounds also can be detected and quantified.

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany