CN
UHPLC Analysis of Sulfa Drugs on Ascentis® Express C18
CONDITIONS
column
Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 μm particles (50813-U)
mobile phase
[A] 0.1% acetic acid; [B] 0.1% acetic acid in acetonitrile; (90:10, A:B)
flow rate
0.8 mL/min
pressure
10720 psi (739 bar)
column temp.
45 °C
detector
UV, 250 nm
injection
0.5 μL
sample
25 μg/mL in 90:10, water:methanol
说明
分析说明
The UHPLC method presented here shows the rapid analysis of the sulfa drugs sulfacetamide, sulfadiazine, sulfathiazole, and sulfamerazine on Ascentis Express 2 μm C18. Analysis time is less than one minute. The sulfa drugs analyzed here all contain a sulfanilamide substructure as active group.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany