GCxGC Analysis of Allergens in Perfume Sample 1 on SLB®-5ms x SLB®-35ms, Non-Polar to Polar Strategy
Materials
related product
1,3,4,6,7,8-Hexahydro-4,6,6,7,8,8-hexamethylcyclopenta[g]-2-benzopyran solution
in diethyl phthalateLinalool
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCoumarin
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland4-(4-Hydroxy-4-methylpentyl)-3-cyclohexene-1-carboxaldehyde
mixture of isomers, analytical standardVanillin
certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, SwitzerlandCONDITIONS
column
(1D) SLB-5ms, 20 m x 0.18 mm I.D., 0.18 μm (28564-U)
column
(2D) SLB-35ms, 5 m x 0.25 mm I.D., 0.25 μm (29804-U)
oven
(1D) 45 °C, 3 °C/min to 230 °C
oven
(2D) 50 °C, 3 °C/min to 230 °C
oven
(modulator) stainless steel accumulation loop measuring 20 cm x 0.71 mm O.D. x 0.51 mm I.D.; modulation period 5.4 sec (accumulation period 4.9 sec; injection period 0.5 sec)
detector
qMS; ionization mode: electron ionization (70 eV); interface and ion source temperatures: 250 °C and 200 °C, respectively; mass range: 40-360 m/z; scan frequency: 25 Hz;
carrier gas
helium, initial inlet pressure: 130.8 kPa; initial auxiliary pressure: 65.2 kPa; in both dimensions the linear velocity was maintained constant
Description
Analysis Note
Data provided by Flavio A. Franchina (1), Mariasora Maimone (2), and Luigi Mondello (1,2) of (1) Chromaleont s.r.l., c/o “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy or (2) “Scienze del Farmaco e Prodotti per la Salute” Department, University of Messina, viale Annunziata, 98168 Messina, Italy.
The chromatogram shown here represents research toward the development of a flow-modulation comprehensive 2D GC/MS (FM GCxGC/MS) method for the determination of recently indicated allergens in fragrances. The detector was a rapid-scanning single quadrupole MS (quadMS). SLB-5ms and SLB-35ms GC columns were used. Mass spectral matching was carried out by using the FFNSC database.
Peak ID:
1. α-Pinene
3. β-Pinene
4. Limonene
7. Terpinolene
8. Linalool
9. Camphor
13. α-Terpineol
17. Linalyl Acetate
20. Geraniol
21. Geranial (Citral component)
24. Hydroxycitronellal
29. Eugenol
39. α-Isomethyl Ionone
47. Tetramethyl Acetyloctahydronaphthalenes
Legal Information
SLB is a registered trademark of Merck KGaA, Darmstadt, Germany