material
glass support, silica gel 60 matrix
feature
binder Organic Polymer, fluorescent indicator: no
packaging
pkg of 25 plates
technique(s)
HPTLC: suitable
layer thickness
100 μm
plate L × W
20 cm × 10 cm
particle size
5-6 μm
pore size
60 Å medium pore diameter
storage temp.
2-30°C
General description
for peptid analysis 20 x 10 cm
Analysis Note
Specific surface area (according to BET; 5-Pt. measurement): 480 - 540 m²/g
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 70 - 120 µm
Deviation of layer thickness per plate: ≤ 20 µm
Aminoacid test
hRf-values
- L-Lysine:
2 - 7
- L-Glutaminic acid: 10 - 18
- L Glutamine: 25 - 35
separation number: ≥ 8.5
Typical value determined on an activated plate (120°C, 15 min)
Eluent: 2-Butanol/ pyridine/ ammonia solution 25%/ water (39/34/10/26 v/v/v/v) + 0.05 mol/L Lithium chloride (1mg/ml)
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 70 - 120 µm
Deviation of layer thickness per plate: ≤ 20 µm
Aminoacid test
hRf-values
- L-Lysine:
2 - 7
- L-Glutaminic acid: 10 - 18
- L Glutamine: 25 - 35
separation number: ≥ 8.5
Typical value determined on an activated plate (120°C, 15 min)
Eluent: 2-Butanol/ pyridine/ ammonia solution 25%/ water (39/34/10/26 v/v/v/v) + 0.05 mol/L Lithium chloride (1mg/ml)