CpGenome直接制备亚硫酸氢盐改性试剂盒（50次反应）

DNA methylation is an important epigenetic mechanism regulating gene silencing, imprinting, embryonic development, and chromosome stability. DNA methylation occurs on the 5 carbon position of cytosine residues mainly within CpG dinucleotides to form 5-methylcytosines (5-mC). The reaction is catalyzed by DNA methyltransferases (DNMTs). 5-methylcytosines residues may also be hydroxylated by TET enzymes to form 5-hydroxymethylcytosine (5-hmC), which has differing roles from 5-mC. EMD Millipore provides robust tools that enable you to not only detect and quantify 5-mC and 5-hmC, but also to accurately distinguish between these modifications.

Unlike other bisulfite modification approaches that start with isolated genomic DNA, the CpGenome™ Direct Prep, and CpGenome™ Direct Prep-96 kits allow simple and efficient bisulfite conversion of DNA directly from your sample. Avoid the need to isolate genomic DNA and perform bisulfite modification of unmethylated cytosines starting with fresh or frozen tissues, cultured cells, whole blood, buffy coat, biopsies, or FFPE (formalin-fixed paraffin-embedded) samples.